منابع مشابه
The clinical usefulness of the measurement of cytokines.
The utilization of accurate and sensitive methods for the measurement of cytokines in body fluids is prerequisite for the proper use of these mediators in clinical practice. Many factors contribute to the complexity of cytokine quantitation. Bioassays historically preceded immunoassays, which are now very popular, but there is a need for standardization. Nevertheless, due to the local effects o...
متن کاملMeasurement of protein cytokines in tissue extracts by enzyme-linked immunosorbent assays: application to lipopolysaccharide-induced differential milieu of cytokines.
OBJECTIVES Determination of protein cytokines in local tissues would help to evaluate their local role in health, sickness behavior and immune-mediated diseases. Therefore, developing a simple quantitative method of protein cytokines in tissues/organs is highly important. METHODS Mouse tissues were collected following intraperitoneal administration of endotoxin-free PBS or lipopolysaccharide....
متن کاملRole of Cytokines in Pathophysiology of Asthma
The worldwide incidence, morbidity and mortality of asthma are increasing dramatically. It is one of the most common disorders encountered in clinical medicine in both children and adults. It affects approximately 5% of the adult population in the western world and its reported incidence is increasing vigorously in many developed nations. A network of a novel mediator known as ‘pleiotropic cyto...
متن کاملMeasurement of Induced Cytokines in AIDS Clinical Trials Using Whole Blood: A Preliminary Report
ROBERT S. WALLIS,* HOWARD M. LEDERMAN, JOHN SPRITZLER, JENNIFER L. DEVERS, DANIEL GEORGES, ADRIANA WEINBERG, SUSAN STEHN, MICHAEL M. LEDERMAN, AND THE ACTG INDUCIBLE CYTOKINES FOCUS GROUP† Department of Medicine, University Hospitals and CWRU School of Medicine, Cleveland, Ohio; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Biostatisti...
متن کاملSimultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity.
BACKGROUND Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, IL-7, IL-8, and interferon (IFN)-gamma have been reported elevated in psoriatic patients. AIM To evaluate serum cytokine profiles in psoriasis patients by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disea...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2012
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.290